Diabetes Autoantibodies Western Blot Kit (Western Blot Method)
- Product description
- Product background
- References cited
Diabetic Autoantibody Western Blot Kit (Western Blot Method)
Product features | |
Easy to operate |
The operation method is simple and easy to learn, no professionals are required |
Joint testing |
Detect multiple antibodies to diabetes, more accurate |
Wide application |
No need for precision instruments, suitable for all kinds of hospitals |
Product performance parameters:
Table 1
General specifications | |
Intended use |
In vitro qualitative detection of autoantibodies against diabetes in human serum provides a reference for the classification, prediction and prevention of diabetes. |
Inspection principle |
Transfer the antigen of DM to the nitrocellulose membrane by gel electrophoresis, and observe the coloring area after serological reaction to judge DM |
Detection time |
The fastest as long as 80min |
Package specifications |
40 servings/box |
Table 2
Reagent components | |
Concentrated washing liquid (10×) |
25mL×1 bottle |
Enzyme-linked reagent |
1mL×1 bottle |
Developer A liquid |
25mL×1 bottle |
Developer B liquid |
25mL×1 bottle |
Stop solution |
25mL×1 bottle |
Sample diluent |
100mL×1 bottle |
Blotting film |
40 servings |
Standard belt |
1 copy |
Kit storage conditions |
Store at 2~8℃ in the dark for 1 year, and the validity period is 6 months after opening |
Table 3
Operating environment | |
Ambient temperature |
20~37℃ |
Relative humidity |
|
Table Four
Accessories and supplies | ||
Shaker |
Washing bottle |
Pipette gun and its matching tips |
Table 5
Reaction system | |
Sera dilution factor and liquid addition volume |
Dilution 101 times, 1 mL of sample diluent for each person and 10uL of serum to be tested |
Reaction temperature |
20~37℃ |
Table 6
Sample conditions | |
Sample type |
Serum |
Sample storage |
Fresh serum should be used for the specimen to be tested. If it is not tested on the same day, it should be stored at 2~8℃; if it is more than 24 hours, it should be stored below -20℃ |
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