Rheumatism Autoantibodies Western Blotting Kit (Western Blotting)
- Product description
- Product background
- References cited
Rheumatism Autoantibody Western Blotting Kit (Western Blotting)
Product Features |
|
Easy to operate |
The operation method is simple and easy to learn, no professionals are required |
Classification detection |
It is more reasonable to detect a variety of rheumatism antibodies and multiple items for joint inspection |
Wide application |
No need for precision instruments, suitable for all kinds of hospitals |
Product performance parameters:
Table 1
General specifications |
|
Intended use |
This kit can be used by clinical laboratories for the qualitative determination of autoantibodies in patients with rheumatism, and provides a reference for the diagnosis and identification of rheumatism. |
Inspection principle |
Transfer the rheumatism antigen to the nitrocellulose membrane by gel electrophoresis, and observe the color area after serological reaction to judge the existence and type of rheumatism |
Detection time |
The fastest as long as 80min |
Package specifications |
40 servings/box |
Table 2
Reagent components |
|
Concentrated washing liquid |
25mL×1 bottle |
Enzyme-linked reagent |
0.5mL×1 bottle |
Developer A liquid |
25mL×1 bottle |
Developer B liquid |
25mL×1 bottle |
Stop solution |
25mL×1 bottle |
Blotting film |
40 servings |
Standard belt |
1 copy |
Kit storage conditions |
Store at 2~8℃ in the dark for 1 year, and the validity period is 6 months after opening |
Table 3
Operating environment |
|
Ambient temperature |
20~37℃ |
Relative humidity |
|
Table Four
Accessories and supplies |
||
Shaker |
Washing bottle |
Pipette gun and its matching tips |
Table 5
Reaction system |
|
Sera dilution factor and liquid addition volume |
Dilution 101 times, 1 mL of washing application solution for each person and 10uL of serum to be tested |
Reaction temperature |
20~37℃ |
Table 6
Sample conditions |
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Sample type |
Serum, is there whole blood? |
Sample storage |
Fresh serum should be used for the specimen to be tested. If it is not tested on the same day, it should be stored at 2~8℃; if it is more than 24 hours, it should be stored below -20℃ |
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